Bacterial wilt of carnation

[1] Previously named Pseudomonas caryophilli,[2] the pathogen is an aerobic gram negative bacteria known for only being capable of entering its host through wounds.

Once inside the host, it colonizes the vascular system and roots causing symptoms such as, internal stem cracking, yellowing of the leaves, wilting, and the development of cankers.

[3] Although there may be an extended period of latency, once plants begin showing symptoms, death typically occurs within 1–2 months.

[citation needed] To make a reliable diagnosis, multiple samples of stems should be examined (young and old) and isolation should be made from the diseased tissues.

The bacterium can be detected by immunofluorescence staining (IFAS) and direct isolation on 96 microwell cell culture plate even in material with latent infection.

When the canker, which contains accumulated yellowish-brown bacterial slime, oozes out via an opening, the bacteria is released to the surrounding soil.

Turf and sphagnum peat have been reported to be a suitable environment for B.caryophylli, and the use of these natural substrates may enhance spread, establishment, and survival of the pathogen.

[7] Current cultural practices and general control measures are very effective in keeping the crop free from the bacterium in protected environments.

In checking for the presence of disease before further planting, induced high temperatures should be employed to accelerate bacterial growth and symptoms.

At an early stage of disease, the “KPV-Metoden” testing method can be used to detect infections present in carnation cuttings.

Its impact areas include the former Yugoslavia, Italy, Serbia, Montenegro, China Taiwan, India, South America, (Brazil, Colombia, and Uruguay), and the United States.

[10] It is because of the devastating effects in crop loss that breeding programs were initiated in 1988 by the National Institute of Floriculture Science (NIFS) Japan in search for resistance.

[10] Bacterial Wilt of Carnation is also defined as an A2 quarantine pest by the European and Mediterranean Plant Protection Organization (EPPO).

Healthy carnation