Charles Tulasne (5 September 1816 – 28 August 1884) was a French physician, mycologist and illustrator born in Langeais in the département of Indre-et-Loire.

Afterwards he worked with his older brother Louis René Tulasne (1815–1885) in the field of mycology.

In addition to assisting his brother with the classification and study of fungi, Charles Tulasne collaborated with Louis on numerous scientific publications.

Regarding the artistic quality of his work, Charles Tulasne is sometimes referred to as "The Audubon of Fungi".

In 1872, Joseph Schröter circumscribed a genus of effused (patch-forming) fungi in the Tulasnellaceae family, Tulasnella was named in Tulasne's honour.