The RS2 region, which measures 2,4 kb, contains genes that control the replication, regulation, and integration of CTXφ.

The first is the toxin-coregulated pilus (TCP), which also aids the bacterium in adhering to the intestinal cell wall.

A similar mechanism is suspected in the injection of the CTXφ genetic material into the V. cholerae cell, though further research is needed to confirm this.

Once the new ssDNA genome is inside the assembled proteins, the CTXφ virion detaches from the EpsD and is free to infect other bacteria.

[4] Recent research suggests that at least two toxins other than CT are produced from genes of the CTXφ genome.

Zot, though absolutely essential for the production of the CTXφ virion, is not actually present in the phage particle.