It stays considerably lower in the trees than the other nocturnal strepsirrhines in its range, and is typically found between 5 and 15 metres above ground.

When confronted by a predator, the Calabar angwantibo will roll up into a ball, but keep its mouth open beneath its armpit.

[citation needed] Calabar angwantibos forage for food alone, but each male's territory overlaps that of several females.

Mating takes place only in the final phase of the female's estrous cycle, and is performed hanging upside-down from a branch.

The female gives birth to a single infant after a gestation period of 131 to 136 days; the young are normally born between January and April.