Later, Cali Zoo's administration started a complete medical collection of each animal's records, which allowed it to have accurate and organized information so it could provide better care.

With these basic necessities satisfied, Cali Zoo could lead its objectives to research and preserve the different Colombian wild species.

The zoo has five sections for different kinds of species: primates, butterflies, ocean and river fish, amphibians and reptiles, and birds.

[2] Mammals at the zoo include giant otters, Neotropical otters, red kangaroos, capybaras, giant anteaters, coppery titis, tufted capuchins, Colombian spider monkeys, Guianan squirrel monkeys, ring-tailed lemurs, collared brown lemurs, meerkats, hamadryas baboons, spotted hyenas, lions, Bengal tigers (including a white tiger), jaguars, pumas, spectacled bears, South American coatis, blackbucks, white-tailed deer, llamas, collared peccaries, mountain tapirs, South American tapirs and Grant's zebras.

Reptiles and amphibians at the zoo include poison dart frogs, gold tegus, central bearded dragons, Burmese pythons, green anacondas, American crocodiles, spectacled caimans and several species of turtle and snake.