[2] Calopogon multiflorus can be found in dry to moist flatwoods with wiregrass, longleaf pine, and saw palmetto.

These longleaf pine savannas were once widespread in southeastern North America,[5] and they burned naturally at least once a decade (see map in fire ecology).

Other species that are found growing nearby in the same habitat are blackjack oak (Quercus marilandica), little gallberry/ink berry (Ilex glabra), slender bluestem (Schizachyrium tenerum), little bluestem (S. scoparium), and savannah meadow beauty (Rhexia alifanus).

[5] It is known to bloom six to eight weeks after a burn, likely benefiting from the lack of competition with other plants, and the nutrients released during a fire.

[3] Although this orchid is known historically in Florida and other managed areas, it is now rare due to fire suppression and conversion of habitat to pine plantations.

These flatwoods can also be protected from bedding, draining, clearcutting, roller-chopping and other soil and hydrology disturbances.