The song has been recorded by many groups and musicians: Blind James Campbell, Bob Dylan, The Band, The Staple Singers, Clara Ward Singers, John Fahey, Roy Acuff, Joan Baez, The Chieftains, Jerry Lee Lewis, Gene Vincent, Ralph Stanley, The Black Crowes, Kristin Hersh, John Lee Hooker, Bill Monroe, the Nitty Gritty Dirt Band, The 13th Floor Elevators, Pentangle, Spacemen 3, Country Joe McDonald, John Statz, Spirit of the West with The Wonder Stuff, Mavis Staples, The Felice Brothers, Johnny Cash, Gregg Allman, the Neville Brothers, Jeff Buckley, Moby, and Agnes Chan.
Almost all cover versions of the song use a straight 4/4 meter throughout, while the Carter Family recording from 1927 uses bars of 3/4 near the end of each verse and twice in the chorus.
In 1988, springing from the Great Hudson River "Clearwater" Revivals (now referred to as Festivals) which Pete Seeger championed from the mid-1960s through the present, the second line of the chorus was rewritten and copyrighted by Cathy Winter, Betsy Rose, Marcia Taylor and Terry Dash as "Rise Up Singing": Will the circle be unbroken, by and by Lord by and by.
I was born down in the valley where the sun refused to shine but now I'm climbing - up to the highlands - Gonna make all those mountains mine.
The song's refrain and lyrics were worked into the track "High & Hurt" by the Danish band Iceage, featured on their 2021 album Seek Shelter.