[1][2] The seat of Spence (SA) was vacant following the resignation of Nick Champion (Labor) on 22 February 2022 to contest the South Australian state election.
A second Senate seat in Western Australia was initially vacant at the close of nominations following the resignation on 15 April 2022 of Ben Small (Liberal), who had discovered that he was ineligible on the grounds of dual citizenship.
Having renounced his New Zealand citizenship, Small was re-appointed on 18 May 2022 and contested the election.
Senators Tim Ayres (Labor), Andrew Bragg (Liberal), Perin Davey (National), Mehreen Faruqi (Greens), Hollie Hughes (Liberal) and Tony Sheldon (Labor) were not up for re-election.
Danny Lim Julie Collins Warren Grzic Guitang Lu William Laing Two seats were up for election.
Robert Lyon (KAP) David Schefe Lindsay Temple Chey Hamilton Lorraine Smith Laurence Quinlivan Karakan Kochardy Peter Rogers Six seats were up for election.
The Centre Alliance had two of their seats up for re-election, although sitting senator Rex Patrick left the party and contested instead for the Rex Patrick Team, whilst senator Stirling Griff ran as the second Independent on the Nick Xenophon group ticket.
Senators Catryna Bilyk (Labor), Carol Brown (Labor), Claire Chandler (Liberal), Richard Colbeck (Liberal), Jacqui Lambie (JLN) and Nick McKim (Greens) were not up for re-election.
Glenn Floyd Allen Ridgeway James Bond Neal Smith Max Dicks Bernardine Atkinson Paul Ross Nat De Francesco Joseph Toscano Tara Tran David Dillon Geraldine Gonsalvez Six seats were up for election.