Three years after defeating Germany and winning the France World Cup for Japan, Tsubasa Oozora moves to Brazil alongside his coach Roberto Hongo in order to play for São Paulo F.C.

Tsubasa intends to help São Paulo win the Rio Cup by overcoming six Brazilian elite teams, including Fluminense, Corinthians, Grêmio, Palmeiras, Santos, and Flamengo.

At the same time, Nankatsu, led by Taro Misaki, along with Shun Nitta and Ryo Ishizaki, competes in the High School Soccer Championship against Kunimi Gakuin, Akita, Tatsunami, Musashi, Furano, and Toho.

Rivals faced in this tournament are well-known characters from the manga including Hiroshi Jito and Mitsuru Sano (Kunimi), the Tachibana twins (Akita), Makoto Soda and Taichi Nakanishi (Tatsunami), Jun Misugi (Musashi), Hikaru Matsuyama (Furano), Kojiro Hyuga, Ken Wakashimazu, and Takeshi Sawada (Toho).

In the last part of the game, Japan takes on Mexico, Italy, Netherlands, Argentina, Germany, and finally Brazil, managed by Tsubasa's former coach, Roberto Hongo.