His interest in medicine began while he was aiding in the care of the wounded after the battle of Germantown, and he made his first studies under the direction of Dr. John Redman.
It was his habit to throw open his house once every week in the winter, and at these gatherings students, citizens, scientists, and travelers met and discussed subjects of interest.
These assemblies, celebrated in the annals of Philadelphia under the title of Wistar parties, were continued long after his death by other residents of that city.
The botanist Thomas Nuttall named the genus Wisteria in his honour (some call it Wistaria but the misspelling is conserved under the International Code of Botanical Nomenclature).
His brother Richard (20 July 1756 – 6 June 1821) was a Philadelphia merchant who built a large four-story store in 1790 where he conducted an iron and hardware business.
During the Revolutionary War, Richard advocated the defense of his property by arms, which resulted in his being disowned by the Society of Friends.
Richard was an inspector of prisons, and was one of the early friends and supporters of the Library Company of Philadelphia and the Pennsylvania Hospital.