Jerusalem, Cyprus and certain other claims and possessions could go to a different heir, and they did, in principle, going to Charles II's sister Yolande Louise.
In 1713, Charles's great-great-great-grandson Victor Amadeus II of Savoy received confirmation to that title from the Kings of Spain and France, who also claimed it.
In response to the riots between Catholic and Protestants within Geneva, Charles launched a surprise attack in July 1534, but his army was beaten back.
[2] A second siege in October 1535 was attempted, and again Charles's army was defeated when forces from Berne arrived to assist Geneva.
Beatrice was both first cousin and sister-in-law of Emperor Charles V. They had nine children, but only one child, Emmanuel Philibert, would reach adulthood: