[1] The natural habitat of Grammostola rosea is the high desert and scrub regions of northern Chile, Bolivia, and Argentina.

[6] Natural habitats of the Chilean rose tarantula have often been disturbed by human activity, industrialization and urbanization, making exact distribution of the species harder to pinpoint.

This tarantula has a diverse diet, including numerous beetles, caterpillars, crickets, cockroaches, grasshoppers, mealworms, silkworms, waxworms and even small lizards and rodents.

[10] The urticating hairs disperse into the air, causing irritation and itching when in contact with predator's skin or eyes.

[10] The urticating hairs also act as sensory structures, helping the spider identify subtle vibrations or changes in pressure.

G. rosea is usually skittish, running away from danger rather than acting defensively, but it may also raise its front legs and present its fangs in preparation to defend itself.

As with the majority of tarantulas from the Americas, it has small, spine-like urticating hairs on its abdomen that it kicks off or releases when threatened as a defense.

[13] The venom of the Chilean rose tarantula contains multiple toxins, which may help it immobilize and digest prey, as well as deter predators.

A specific peptide found in this venom, termed GsMTx4 (Grammostola rosea Mechanotoxin 4) has been shown to inhibit mechanosensitive ion channels in living cells.