[3][4] Barnekow spent nearly 16 years of his early life travelling throughout Europe and the Middle East, including a three-year journey in the Holy Land and Egypt.
[5] Probably in 1591 he gave his autograph to Dietrich Bevernest, writing a Spanish proverb, Qui no ci cança, nunca alcança, meaning "those who never grow weary will achieve".
King James VI had an audience with them on 15 July, and after a few days arranged for them to lodge in private houses in Lothian because he could not afford to host them.
[8] Barnekow and his colleague Steen Bille brought necklaces for Queen Anne and Prince Henry, and King James gave them gold chains, which were heavier than those made for the ambassadors from the dukes of Mecklenburg and Brunswick.
He brought a jewel from the Cardinal that depicted the Crucifixion made of gold, crystal, and bone, which King James gave to Queen Anne.
[12] In September 1597, Barnekow and Arild Huitfeldt (1546–1609) were envoys to London and lodged in Fenchurch Street in the house of Alderman Houghton or the Customer Master Smith.
He acquired Tølløsegård (Tølløse Slot) jointly with his brother Jens Barnekow, in 1592 from the estate of Mette Rosenkrantz (1533-1588), widow of Peder Oxe.