The original chapel of St Bartholomew, which stood on the site of the present church, was documented by inquisitor Pietro Dusina on his apostolic visit to Malta in 1575.

With the growing population of the village, the Bishop of Malta Tomás Gargallo created the new parish of Għargħur.

The site of the chapel of St Bartholomew was chosen for the new church which commenced in 1610 on plans by Tommaso Dingli.

The church was consecrated by Archbishop Paul Alphéran de Bussan on April 22, 1736.

Other paintings in the quire depict the Last supper and the Nativity of Jesus, both the work of Francesco Zahra.