A city of regional significance (Ukrainian: місто обласного значення, romanized: misto oblasnoho znachennia) in Ukraine was a type of second-level administrative division or municipality, the other type being raions (districts).

It was abolished in a 2020 reform that merged raions together and integrated the city municipalities into them.

A city was usually granted the status for being an economic and cultural center that has a developed industry, utilities, a considerable amount of state provided housing, and a population of over 50,000, and if for further economic and social development of a city it was appropriate to establish a direct management by regional organizations.

In some cases, a city with a population of less than 50,000 could be a city of regional significance if it had industrial, social-cultural, and historical significance, or a propensity for further economical and social development and population increase.

These exceptions were usually granted on a decision of the Verkhovna Rada (the Supreme Council of Ukraine).