[1] This line provides service from Federal Triangle in Washington, D.C., to the neighborhood of Culmore in Fairfax County.

16F operated during peak hours only, serving to Pentagon Station in the morning, and the neighborhood of Culmore in the afternoon.

[9] From the three options, the 16F will operate on limited stops on Columbia Pike, like its original route, but will serve in all stops in Washington D.C.[9] Beginning on December 19, 2010, additional 16F trips will operate between Pentagon station and Federal Triangle station replacing routes 13A and 13B from the National Airport–Pentagon–Washington Line.

Afternoon eastbound trips runs between Pentagon station and Federal Triangle.

Although, select rush hour trips will run on short intervals on both directions.

According to performance measure it goes as the following for WMATA:[12] On June 24, 2018, major bus changes occurred in Columbia Pike.

The 16X was eliminated due to multiple routes running through Columbia Pike in Arlington.