In March 1727, with Giuseppe Rossi as an assistant, Giaquinto opened an independent studio near the Ponte Sisto, in the parish of Saint Giovanni of the Malva in Rome.
In 1731, he received a prestigious commission, to execute frescoes in the church of San Nicola dei Lorenesi: Saint Nicholas water gush from cliff, three theologic and cardinal Virtues, and in the cupola Paradise.
In 1733, the architect and artistic director for the House of Savoy, Filippo Juvarra, invited Giaquinto to come to Turin, where he painted an altarpiece of Saint John Nepomuk.
In 1738 Giaquinto returned to Rome, and during the next year, he executed in fresco an Assumption of the Virgin for the church of Rocca di Papa, a commission for a relative of Pope Alexander VIII Ottoboni.
In 1740, Giaquinto became a member of the Academy of Saint Luke and donated his sketch of Immaculate Conception with Elias the prophet for the Turinese church of the Carmine, a canvas commissioned by Marquis Giuseppe Turinetti di Priero, which finally reached Turin in 1741.