The road's western terminus was at an intersection with NY 50 and County Route 58 (CR 58) in the hamlet of Burnt Hills.

Its eastern terminus was at a junction with NY 146A near the hamlet of Ballston Lake.

NY 339 then veered slightly towards the northeast, intersecting with several local roads as it approached Ballston Lake.

[2] It remained unchanged up through 1973, at which time NY 339 was removed from the state highway system.

[3] The former routing of NY 339 is now maintained by Saratoga County as CR 339.