Cowboy Serenade is a 1942 American Western film directed by William Morgan and starring Gene Autry, Smiley Burnette, and Fay McKenzie.

Written by Olive Cooper, the film is about a singing cowboy and cattleman who goes after a gambling ring after they fleece the cattlemen association's representative of their cattle.

The next day, while traveling to Hays City with the cattle, Jimmy is persuaded to join a poker game by two crooked gamblers, Dixie Trambeau (Tristram Coffin) and Joe Crowley (John Berkes), who fleece the naive cattleman and force him at gun point to turn over the power of attorney authorizing them to sell the cattle.

At Hays City, Gene and Frog meet with District Attorney Stevens (Forbes Murray), who tells them about Trambeau's crooked gambling ring, which is responsible for fleecing many ranchers.

Later, Gene and Frog discover the gamblers' hideout, but Trambeau and Crowley are able to escape with the help of Priscilla and the Locks' Chinese servant and ride back to the ranch.