The Women's time trial B road cycling event at the 2016 Summer Paralympics took place on the afternoon of 14 September at Flamengo Park, Pontal.

14 riders (with pilots) competed over two laps of a fifteen kilometre course.

[1] The B category is for cyclists with visual impairment.

Sighted guides act as pilots in these events, which take place on tandem bikes.

Women's road time trial B.