The women's team pursuit competition of the cycling events at the 2023 Pan American Games was held on 24 and 25 October at the Velódromo Peñalolén in Santiago, Chile.

[1][2] Prior to this competition, the existing world and Games records were as follows: All times are local (UTC–3) All teams advanced to the first round.

[3] First round heats were held as follows: Heat 1: 5th v 6th fastest Heat 2: 2nd v 3rd fastest Heat 3: 1st v 4th fastest The winners of heats 2 and 3 proceeded to the gold medal race.

The remaining four teams were ranked on time, from which the top two proceeded to the bronze medal race.

[4]