The major differences between the two are the frame geometry, and the wider clearances that cyclo-cross bikes have for their larger tires and mud and other debris that they accumulate.
Cyclo-cross courses are held on a variety of terrain, including grass, mud, sand and paved surfaces.
At first the frames were usually made of steel; nowadays, aluminum and carbon fibre are more common, at least in regard to cyclo-cross bikes built for racing.
Cyclo-cross racers may lift or carry their bikes as many as 30 times in one 60 minute race, increasing the desire for a lightweight bicycle.
Cyclo-cross frames require clearance for slightly wider (generally 30–34 mm) tires and the debris and mud that is picked up by them.
Typically, the frame geometry is somewhat more relaxed than that of a road bike, not as upright in the seat tube, which allows for more stability in soft ground.
Also, the wheelbase can be a bit longer, which provides a more planted feeling when using aft body weighting over rough terrain, and can help keep the rider's feet from contacting the front wheel during a tight turn.
Choices of equipment tend more towards the idiosyncratic than in road racing; for example single-speed bicycles also have some popularity due to the advantage of mechanical simplicity in the often very muddy conditions and the fringe nature of the sport.
In general, with a change of tires and gearing a cyclo-cross bike can double as a perfectly adequate road racing machine.
However, most cyclo-cross racers prefer clipless mountain bike pedals for their easy dual-sided entry and mud-shedding abilities.
Tubular tires also offer a weight saving and the ability to be ridden on even when flat so that a racer can reach the pits for a replacement.
However, since 2005 several tubular manufacturers have designed more modern style tread patterns, namely Dugast with its Rhino, Tufo with its Flexus and Challenge with its Grifo and Fango.
Dry tires tend to have much smaller, closely spaced tread such as the diamond pattern for low rolling resistance.
Wet weather tires have larger and more widely spaced knobs to aid in grip and mud shedding.
It is common for racers even at an amateur level to have a pair of race bikes (one to ride and one in the pits as a back-up) and several wheels to choose from.
More recently, tubeless clincher tires have become popular after their successful presence on mountain bikes for the last several years.