In the episode, frustrated with men, Mrs. Garrison makes the boys write an essay on The Old Man and the Sea.
[1] When the episode begins, Mrs. Garrison storms into her classroom enraged over a failed date, and takes her anger out on her male students with an essay assignment over the weekend, making them read The Old Man and the Sea by Ernest Hemingway in its entirety.
At Cartman's urging, they hire local Mexican laborers looking for work to read the book and write their essays for them.
The next day, when the boys try to explain themselves to Mrs. Garrison about their essay, she happily gives them more time to work, announcing she is a lesbian, which the class is highly supportive of.
Later, Xerxes arrives, and attempts to reason with Mrs. Garrison, even offering her the job of running the club when he takes control.
[2] The lesbians' fight against the Persians, with Mrs. Garrison kicking the messenger, Rauf Xerxes's physical appearance, and a plethora of slow-motion sequences, are references to the Zack Snyder film 300.
The film that is parodied, 300, portrays the defense of the Greek city-states by Spartan warriors at the Battle of Thermopylae.
The bar "Les Bos" can be compared to the Greek island of Lesbos, from which the word Lesbian is derived.