Daemen is a member of the East Coast Conference (ECC), competing at the Division II level of the National Collegiate Athletic Association (NCAA).

Women's sports include basketball, bowling, cross country, indoor and outdoor track, soccer, tennis, triathlon, and volleyball.

The Wildcats compete mainly in NCAA Division II and are part of the East Coast Conference (ECC) since the 2013–14 school year.

They formerly competed in the National Association of Intercollegiate Athletics (NAIA) as part of the now-defunct American Midwest Conference (AMC) from 2001–02 to 2011–12; as well as an Independent during its transition to D-II in the 2012–13 school year.

The Wildcats joined the D-I Northeast Conference as an associate member starting with the 2023 season (2022–23 school year).