The Mercedes-Benz T80 land speed record car, designed by aircraft engineer Josef Mickl with assistance from Ferdinand Porsche and top German Grand Prix racing driver Hans Stuck, incorporated the third prototype DB 603.

It was set up for the land speed record run attempt to operate on an exotic fuel mix based on a 63% methanol, 16% benzene and 12% ethanol content, with minor percentages of acetone, nitrobenzene, avgas and ether.

Adding to the power output was a pioneering form of the Luftwaffe's later MW 50 methanol/water injection boost, and was tuned to 3,000 PS (2,959 hp, 2,207 kW)— enough, it was believed, to propel the aerodynamic three-axle T80 up to 750 km/h (466 mph) on a specially-prepared, nearly 10 km (6.2 mi) length stretch of the roughly north–south oriented Autobahn Berlin — Halle/Leipzig, which passed close to the east side of Dessau (now part of the modern A9 Autobahn) and with the actual length's location due south of Dessau, reworked to be 25 m (82 ft) wide with a paved-over median, for the record to be set in January 1940 during Rekord Woche (Record/Speed Week).

The Dornier Do 217M and -N medium bomber and night fighter subtypes powered by inline engines, and the enormous sixty-metre wingspan, six-engined Blohm & Voss BV 238 flying boat prototype, essentially had their DB 603 powerplants installed within what appeared to be the same unitized complete engine/cowl/radiator assembly as a complete unit-replaceable "power system" for twin and multi-engined aircraft — this particular design featured a "chin"-style radiator installation directly beneath the crankcase, and the oil cooler placed on the dorsal portion of the installation for the earlier examples, as the BV 238 had no visible upper-cowl openings for engine cooling of any sort for its half-dozen unitized DB 603s.

The characteristic portside-cowl supercharger intake for Daimler-Benz inverted V12s was usually accommodated away from the nacelle's sheetmetal itself for the Heinkel/DB 603 unitized engine package, most often within the airframe's wing panel design.