The section between Riedstadt-Goddelau and Biblis, which is now considered part of the Mannheim–Frankfurt railway, is of great importance for long-distance passenger services and rail freight traffic.

The last section from Biblis to Worms is used by regional passenger services and rail freight traffic.

[2] On 29 May 1869, the line from Darmstadt via Riedstadt-Goddelau and Biblis to Rosengarten station, on the opposite side of the Rhine from Worms, was opened by the Prime Minister of the Grand Duchy of Hesse, Reinhard Carl Friedrich von Dalwigk.

A siding to the industrial area of Weiterstadt-Riedbahn still exists on the largely disused section between Darmstadt Hauptbahnhof and Riedstadt-Goddelau and it is occasionally used by freight traffic.

In addition, the RE 70, a Regional-Express service, runs from Frankfurt via Groß Gerau-Dornberg, Riedstadt-Goddelau, Biblis and Lampertheim to Mannheim.