The Darwin Falls Wilderness is a protected area in the northern Mojave Desert adjacent to Death Valley National Park.

[4] Though the wilderness is traditionally considered to be within the northern Mojave Desert, some classifications acknowledge floristic affinities with the colder deserts to the north and consider this the Southeastern Great Basin Ecoregion.

[5] A desert scrub community is common in the wilderness with Joshua tree woodlands at higher elevations.

The lowest region of the wilderness area is to the east in Darwin Canyon, which then descends into Death Valley National Park where the spring-fed falls are located less than 1 mile (1.6 km) from the wilderness boundary.

Darwin Falls is in a narrow, shaded gorge where perennial flow and pools allow for a riparian habitat uncommon in the Mojave Desert.