He guest-starred in the television series Law & Order: Criminal Intent as Ricky Moss in the episode Neighborhood Watch, and he appeared on Army Wives.
In 2009, he appeared in five episodes of the drama series Rescue Me as the adult Connor Gavin.
In July 2010, Call joined the cast of The CW's young-adult teen drama series Gossip Girl as Ben Donovan, who is connected to two other then-new characters—Juliet Sharp (Katie Cassidy) and Colin Forrester (Samuel Page).
Call portrayed the recurring role of Nick Lane, on the Fox science-fiction series Fringe.
The character made his first appearance in the first season episode "Bad Dreams", which was critically lauded.