He was the son of Portuguese King Peter I and a Galician noblewoman named Inês de Castro who had arrived in Portugal as a maid of princess Constance of Castile, recently married to Pedro I (Heir Prince at the time).
Other opinions refer that after the death of Inês de Castro, ordered by Pedro's father King Afonso IV of Portugal, the Prince after inheriting the throne admitted that he had married Inês secretly, and because of that she was a lawful Queen of Portugal.
After the death of his mother Denis left Portugal to Castile with his brother but during the 1383–85 Crisis he was one of the claimants to the throne along with Beatrice of Portugal (his niece), John, Master of Aviz (his half-brother) and John (his brother).
Although he managed to be acclaimed King in the city of Santarém his connections with Castile eventually lost him the throne and at the time of the Battle of Aljubarrota he was already out of the country like his brother John.
Lady of Cifuentes, born c. 1360, illegitimate daughter of Henry II of Castile by Juana, 1st?