[1] It is highly adapted to vertical clinging and leaping, like other tarsiers, and has a strict live animal diet, consisting mostly of insects.

[6] Dian's tarsier is found in the primary rainforest[6] of the lower mountains of central Sulawesi.

[8] It can also be found in habitat modified (disturbed) by humans such as secondary forests with logging, agroforestry or intensive agriculture.

Before dawn, duet vocalisation signals to other members of the group to return to the sleeping site.

[4] Dian's tarsiers can travel more than 100 meters to reach their sleeping site in under 15 minutes before dawn.

The pitch lowers towards the middle of the song where most commonly a trill will be made, other options include long notes.

Although this species is also able to sleep in bamboo stands and dense shrubbery which are more common in degraded habitats.

Sleeping sites are typically located on the boundary of the home range, possibly to renew the scent of animals on their territory.

Other commercial avenues used on this land include: bamboo and rattan logging, and plantation of cash crops, such as cocoa and coffee, all plants that are not native to the area.

Researchers hypothesize that this is due to the increased number of insects in slightly disturbed habitat.

The largest home ranges are in the most disturbed habitats, these also have the least insects, likely due to the use of pesticides in plantations.

[10] Females typically cover more of their home range in one night in low and moderate levels of disturbance in their habitats.