The current core cast of Die-Nasty features Delia Barnett, Belinda Cornish, Tom Edwards, Vincent Forcier, Jesse Gervais, Kristi Hansen, Jason Hardwick, Wayne Jones, Mark Meer, and Stephanie Wolfe.
Previous cast members include Matt Alden, Dana Andersen, Shannon Blanchet, Leona Brausen, Peter Brown, Chris Craddock, Jeff Haslam, Kory Mathewson, Chantal Perron, Cathleen Rootsaert, Sheri Somerville, Davina Stewart and Donovan Workun.
In the course of its thirty-season run, Die-Nasty has welcomed many prominent guest stars, including Joe Flaherty, Mump and Smoot, Robin Duke, Mark McKinney, Alan Tudyk and Mike Myers.
Every September, Die-Nasty hosts the annual Soap-A-Thon — currently a 50-hour marathon of continuous improv, which features many performers, technical crew and volunteers going the entire weekend without sleep.
In 2005, then-director Dana Andersen exported the company's annual Soap-A-Thon to England, working with legendary British theatre artist Ken Campbell to produce a 36-hour-long soap opera in London.