The Diocese of Darjeeling is a Latin Church ecclesiastical jurisdiction or diocese of the Catholic Church in India.

It is a suffragan in the ecclesiastical province of the metropolitan Archdiocese of Calcutta, yet depends on the missionary Dicastery for Evangelization.

It includes within its territory the independent Himalayan country of Bhutan, where Christianity is practiced by a tiny minority and proselytism is forbidden.

The cathedral episcopal see is the Marian Immaculate Conception Cathedral, in Darjeeling, West Bengal state, India.

New data from the Annuario Pontifico 2022 reveals as of 2021, it pastorally served 37,320 Catholics (2.4% of 1,535,370 total) on 9,521 km2 in 60 parishes and missions with 147 priests (83 diocesan, 64 religious), 384 lay religious (98 brothers, 286 sisters).