On its apical surface (lumen side), cells of the DCT have a thiazide-sensitive Na-Cl cotransporter and are permeable to Ca, via the TRPV5 channel.

Thiazide diuretics inhibit Na+/Cl− reabsorption from the DCT by blocking the thiazide-sensitive Na-Cl cotransporter.

DCT can be recognized by its numerous mitochondria (even though it is not as much as PCT), basal enfoldings and lateral membrane interdigitations with neighboring cells.

The point where DCT contacts the cortical thick ascending limb of the loop of Henle is called macula densa.

It has tightly packed columnar cells which display reversed polarity and may monitor the osmolarity of blood.