It has two or three linear leaves and up to five yellow flowers with dark red markings.

There are two smooth calli ridges outlined with red near the mid-line of the labellum.

[6] The specific epithet (septentrionalis) means "north" or "northern", referring to the distribution of the species.

[4] Northern bee orchid grows in winter-wet shrubland between Regans Ford and areas inland from Kalbarri in the Avon Wheatbelt, Geraldton Sandplains, Jarrah Forest, Swan Coastal Plain bioregions of south-western Western Australia.

[2][3][4] Diuris septentrionalis is listed as "not threatened" by the Western Australian Government Department of Biodiversity, Conservation and Attractions.