The building was originally constructed in 1929 in Old Town Prague, with art deco designs by Emil Susicky and Pavlo Sydow.

In 2002, floods from the Vltava river damaged the theatre requiring reconstruction and allowing for modernization, some original features from the 1930s remain.

Divadlo v Dlouhé was nominated several times for Alfréd Radok Award in the category Theatre of the Year.

Grabbe's Don Juan and Faust and Sestra Úzkost won the category Performance of the Year.

The most prominent actors are Miroslav Táborský, Jan Vondráček, Vlastimil Zavřel, Jaroslava Pokorná and Klára Sedláčková.