Dmitri Sennikov was born in Leningrad (now Saint Petersburg) and graduated from Zenit football school.

However, Sennikov failed to become a first-team regular and was loaned to Shinnik Yaroslavl after half a season.

In 2000, Sennikov returned to Moscow, having accepted an offer from Lokomotiv, a team for which he went on to make more than 200 appearances in all competitions.

He made his final appearance for the team on 12 October 2005, in a decisive 2006 FIFA World Cup qualification away match against Slovakia, which resulted in a goalless draw and subsequent elimination of Russia from the competition.

At Lokomotiv Moscow, under long-term manager Yuri Syomin, Sennikov usually played on the left of a back-three, despite being right-footed.