In 2022, Rolling Stone ranked "Do Ya Wanna Funk" number 179 in their list of 200 Greatest Dance Songs of All Time.

[6] The song is heard throughout the entire scene where Billy Ray Valentine hosts a party in Trading Places, a comedy movie starring Eddie Murphy as Billy Ray Valentine and Dan Aykroyd as Louis Winthorp III.

The song is covered by Sandra Bernhard in the 1990 film version of her stage show, Without You I'm Nothing.

It is also featured in the 2021 Channel 4 TV series It's a Sin during a club scene whilst Ritchie explains the various theories around the origins of HIV.

The song appeared in the "lip sync for the crown" segment of the season 8 finale of RuPaul's Drag Race: All Stars where contestants and finalists Jimbo and Kandy Muse performed it to impress RuPaul and ultimately win the competition.