My darling I am dreaming of the days gone by, When you and I were sweethearts beneath the summer sky; Your hair has turned to silver, the gold has faded too; But still I will remember, where I first met you.

The old mill wheel is silent and has fallen down, The old oak tree has withered and lies there on the ground; While you and I are sweethearts the same as days of yore; Although we've been together, forty years and more.

In the middle section of his song "We Will All Go Together When We Go," Tom Lehrer parodied "Down By the Old Mill Stream" with a stride piano, singing the following words in reference to a nuclear holocaust: "Down by the old Mael-strom/ There'll be a storm before the Calm."

Redd Foxx and LaWanda Page sing a duet version of "Down By The Old Mill Stream" in the Sanford and Son episode "My Fair Esther".

Sheila Florence as Lizzie Birdsworth sings the chorus of "Down By The Old Mill Stream" in Episode 295 of the Australian TV series Prisoner in 1982.

In the 2014 video game "Skylanders: Trap Team, during Pain-Yatta's villain quest, the Gecko Chorus can be heard singing "Down by the Old Mill Stream"