Dr. William Rainey Holt House, also known as The Homestead, is a historic home located at 408 South Main Street Lexington, Davidson County, North Carolina.

The house was built in 1834, and is a two-story, three-bay, Greek Revival style frame dwelling with a hipped roof.

Following the war, Holt spent an increasing amount of time at his plantation Linwood, located southwest of Lexington, where he operated a scientific farm on his 1,600 acres (6.5 km2).

As president of the North Carolina Agricultural Society, Holt was among the first to introduce purebred breeds of livestock to the state.

This article about a property in Davidson County, North Carolina on the National Register of Historic Places is a stub.