It was first mentioned in historical sources in 1694, when it served as a refuge for monks from the nearby Tvrdoš Monastery which was then destroyed by Venetians during the Morean War.
That year the see of the Serbian Orthodox Eparchy of Zahumlje and Herzegovina was transferred from Tvrdoš to Duži, where it remained until 1777, when the see was relocated to Mostar.
[1] During the latter half of the 19th century, the monks of Duži supported the uprisings of Herzegovinian Serbs against the Ottomans, who therefore damaged and looted the monastery in 1858, 1861, and 1877.
A bronze plaque was placed in the renovated church, with text in Serbian expressing gratitude to Austrian Emperor Franz Joseph.
[1] After World War I, the monastery was inhabited by Russian monks who fled from Russia in the wake of the October Revolution.