The Dubuque Spartans football team represents the University of Dubuque in college football at the NCAA Division III level.

The Spartans are members of the American Rivers Conference (ARC), fielding its team in the ARC since 1929 when it was the Iowa Intercollegiate Athletic Conference (IIAC).

The Spartans play their home games at Chalmers Field in Dubuque, Iowa.

[2] Their head coach is Ryan Maiuri, who took over the position December 28,2023.

† Co-champions The Spartans have appeared in the Division III playoffs four times with an overall record of 0–4.