On 6 January 1742 she married Prince Augustus William of Prussia, second son of King Frederick William I of Prussia and Sophia Dorothea of Hanover.

Prince Augustus William was a younger brother of the reigning Frederick the Great, whose spouse, Luise's own sister, gave him no children.

In her widowhood, she was given the Crown Prince's Palace in Berlin.

Her older sister was Queen Elisabeth Christine of Prussia, wife of her brother-in-law Frederick the Great.

She was also the sibling of Queen Juliana Maria of Denmark and Norway and Charles I, Duke of Brunswick-Wolfenbüttel.