The Dudhwa Tiger Reserve is a protected area in Uttar Pradesh that stretches mainly across the Lakhimpur Kheri and Bahraich districts and comprises the Dudhwa National Park, Kishanpur Wildlife Sanctuary and Katarniaghat Wildlife Sanctuary.

Three large forested areas are extant within the reserve, although most of the surrounding landscape is agricultural.

It shares the north-eastern boundary with Nepal, which is defined to a large extent by the Mohana River.

[3] In July 1976, Billy Arjan Singh acquired a tiger cub named Tara from Twycross Zoo in the United Kingdom, hand reared her and later reintroduced her to the wild in the Dudhwa National Park with the permission of India's then Prime Minister Indira Gandhi.

Billy Arjan Singh sent hair samples of tigers from the national park to the Centre for Cellular and Molecular Biology in Hyderabad where the samples were analysed using mitochondrial sequence analysis.