The team was formed out of Duisburger TfE on 22 March 1900 on the initiative of Gottfried Hinz, who would later go on to become president of the German Football Association (Deutscher Fußball Bund).

A last-place result that season ensured the team would not be part of the newly formed sixteen-team Bundesliga, Germany's first professional league, going on to play instead in the Regionalliga West (II).

TuS 48/99 then made its own appearance in the Gauliga earning a second-place finish in the 1936–37 season, only a single point behind division winners TSV Fortuna Düsseldorf.

After their last place finish in 1962 and fall out of first division play, DSV joined TuS 48/99 to form Eintracht Duisburg the following season in a failed attempt to revive the fortunes of these two sides.

By the late 70s the combined club had fallen to the Landesliga Niederrhein (IV) and by the turn of the millennium they were toiling in the Kreisliga B Duisburg (VIII).