The line runs parallel with the Finow Canal at first and then swings to the southeast after Niederfinow station.

The latter planned to build its line as a direct link between the Upper Silesian coal basin and the Baltic Sea.

[2] After the nationalisation of the BStE in 1879, the entire line was transferred to the Prussian state railways as part of the Royal Division of the Berlin-Stettin Railway (Königlichen Direktion der Berlin-Stettiner Eisenbahn, called Königliche Eisenbahndirektion Stettin from 1905).

At the beginning of the 20th century, plans were developed to establish a separate marshalling yard because of limited space in Frankfurt station.

After much discussion about the location of the new yard, it was eventually decided to build it on the Eberswalde line between Frankfurt and Booßen.

In 1926, a railcar shuttle service was established between Frankfurt and Booßen, serving in particular the extensive facilities of the marshalling yard.

For the service, popularly called the Pendel (pendulum), a separate platform was created in Frankfurt station; it was about the length of a modern tram stop.

The services running to and from Eberswalde did not stop at the three intermediate stations of Paulinenhof, Simonsmühle and Gronenfelde, which were served by the shuttle trains.

During the fighting around Berlin at the end of the Second World War in 1945 the line was in the main combat zone, notably of the Battle of the Seelow Heights.

from the 1960s, the line was used by direct express trains from Angermünde via Bad Freienwalde to Frankfurt (Oder), sometimes running further south.

In addition there six pairs of stopping trains ran over the whole line, with some additional services between Eberswalde and Bad Freienwalde or Wriezen and trains ran between Kietz (now Küstrin-Kietz) and Frankfurt, which following the dismantling of the direct route in 1945 took the Eberswalde line to Booßen.

The southern part of the route has continued to be classified as a main line and was upgraded to a speed of 100 km/h.

In the 1993/1994 timetable, services increased to three express trains from Eberswalde to Frankfurt, stopping at Bad Freienwalde, Wriezen, Werbig and Seelow, with two of them continuing to Dresden.

[3] At the timetable change on 14 December 2014 the Niederbarnimer Eisenbahn took over the operation of passengers services on the route, using Stadler Regio-Shuttle RS1 DMUs.