In bioinformatics, EcoCyc is a biological database for the bacterium Escherichia coli K-12.

EcoCyc contains written summaries of E. coli genes, distilled from over 36,000 scientific articles.

EcoCyc is also a description of the genome and cellular networks of E. coli that supports scientists to carry out computational analyses.

Database objects also describe molecular interactions, including metabolic pathways, transport events, and the regulation of gene expression.

[citation needed] EcoCyc can be accessed through the EcoCyc web site, as a set of downloadable files, and in conjunction with the Pathway Tools software that can be installed locally on Macintosh, PC/Windows, and PC/Linux computers.