Eigen-ji (永源寺) is a Buddhist temple in the Eigenji-Takano neighborhood of the city of Higashiōmi, Shiga Prefecture, Japan.

It is the head temple of one of the 14 autonomous branches of the Rinzai school of Japanese Zen Eigen-ji was founded in 1361 by the famous poet and roshi Jakushitsu Genkō under the sponsorship of the shugo of Ōmi Province, Sasaki Ujinori.

According to a tradition in Inabe city in Mie Prefecture, a priest from Eigen-ji escaped over the Suzuka Mountains into Ise Province bearing the temple's treasures when the temple was destroyed by Takigawa Kazumasa on orders of Oda Nobunaga in 1570; however, there are no records of such an event at Eigen-ji itself.

During the Edo period, the temple was revived by an abbot named Isshi Bunshu from Myōshin-ji in Kyoto, under the sponsorship of Emperor Go-Mizunoo, his consort Tofukumon-in and Hikone Domain.

[1][2] The temple is a noted spot for hanami in spring, with a variant of the sakura known as the "Eigenji-zakura", and for viewing of maple leaves in the autumn.