Audley's television appearances include those in I Love Lucy, The Dick Van Dyke Show, Mister Ed, Hazel, The Beverly Hillbillies, Pistols 'n' Petticoats, and My Three Sons.

[4] Her other stage appearances included On Call (1928–1929);[5] Pigeons and People;[5] Thunder on the Left (both 1933); Kill That Story; Ladies' Money (both 1934); Susan and God (1937–1938; 1943); and In Bed We Cry (1944).

[citation needed] Audley's onscreen appearance was an uncredited role as a parole board member in the 1949 noir film The Story of Molly X starring June Havoc.

[citation needed] Other film appearances followed, including: Pretty Baby (1950); Gambling House (1951); Cell 2455, Death Row (1955); The Unguarded Moment; Full of Life (both 1956); Spoilers of the Forest (1957); Home Before Dark (1958); an uncredited cameo as the mother and slain victim of suspect Jack Graham in The FBI Story (1959); The Second Time Around (1961); and Hook, Line and Sinker (1969).

Audley had recurring roles on The Dick Van Dyke Show as the P.T.A head Mrs. Billings, on The Beverly Hillbillies as the school headmistress Mrs. Potts, on Mister Ed as Wilbur Post's aunt Martha, on Pistols 'n' Petticoats as Mrs. Teaseley,[10] on My Three Sons as Mrs. Vincent, and on Green Acres as Oliver Douglas's disapproving mother, Eunice Douglas, despite being only five months older than actor Eddie Albert who played the role of her son.