1519 – d. 27 September 1547) was the third child and second daughter of Charles Brandon, 1st Duke of Suffolk and Princess Mary Tudor, the Dowager Queen consort of France.

[4][5][6][7] In January 1536, Eleanor was designated the chief mourner for the funeral service of Catherine of Aragon, first Queen consort of Henry VIII, at Peterborough Cathedral.

[8] There is not much known about her later life and she left only one letter: "Dear heart, After my most hearty commendations, this shall be to certify you that since your departure from me I have been very sick and at this present my water is very red, whereby I suppose I have the jaundice and the ague both, for I have none abide [no appetite for] meat and I have such pains in my side and towards my back as I had at Brougham, where it began with me first.

Wherefore I desire you to help me to a physician and that this bearer my bring him with him, for now in the beginning I trust I may have good remedy, and the longer it is delayed, the worse it will be.

Hence the later addition and erroneous use of the arms here suggests that the identity of the portrait was already unclear only two or three generations after it was painted, a situation by no means unusual amid the frequent early deaths, multiple marriages, and shifting alliances and fortunes of the most powerful families of the Tudor era.

"[12] In verifying any such portraits represented as being of Lady Eleanor, it may be useful to know, "when her tomb in Skipton Church was disturbed, in the seventeenth century, her skeleton, which was in perfect condition, proved her to have been 'very tall and large boned,'".