Toufexis acted in small television roles at first, eventually playing Morton in the made-for-television film The Five People You Meet in Heaven, based on a book by Mitch Albom.

He has since appeared on dozens of television shows, including Criminal Minds, The Listener, Painkiller Jane, Flashpoint, Flash Gordon, Stargate Atlantis, Lost Girl, and Houdini & Doyle.

[3] Adam Jensen is the main character in Deus Ex: Human Revolution; Toufexis' wife, Michelle Boback,[1] had a supporting role in this game as scientist Megan Reed.

However, he was replaced by another voice actor, Gianpaolo Venuta, after he worked on the role for two years, as the publisher of the game did not want players to confuse Brody with Jensen.

[7] On February 28, 2018, Toufexis announced on Twitter that he was voicing a character in the new BattleTech video game by Harebrained Schemes, stating that he had sought out a role and was accepted.